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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in several tumors, which results in chromosome instability, and thus might lead to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical position in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants were being described but specific nucleotide sequence data just isn't yet out there.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide normally contains the epitope recognized with the antibody.
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This guidebook outlines even further possible optimization of mobile and immunoassay parameters to make sure the best possible final results are...
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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to guarantee the absolute best results are...
Antibodies sure to the blocking peptide not bind to the epitope to the target protein. By evaluating the staining from the blocked antibody as opposed to the antibody alone, one can see which staining is precise.
Target Facts Cyclin E belongs towards the highly conserved cyclin loved ones, whose users are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation styles which add to the temporal coordination of each mitotic event. Cyclin E types a complex with and functions as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary which is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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